abstract：:Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi org...

journal_title：Parasitology research

authors： Zweygarth E,van Niekerk CJ,de Waal DT

更新日期：1999-05-01 00:00:00

abstract：:Trichinella spiralis infection results in the transformation of muscle cells into a new, non-muscular cell called the nurse cell, and the nurse cell-muscle larva complex is finally created. To investigate whether T. spiralis infectivity is NBL age-dependent, five groups of synchronous newborn larvae (sNBL) were obtain...

journal_title：Parasitology research

authors： Wranicz MJ,Cabaj W,Moskwa B

更新日期：1999-04-01 00:00:00

abstract：:A clinical field trial was performed to evaluate the efficacy of imidacloprid, the active ingredient of Advantage 10% Spot-On, against natural lice infestations on dogs. Imidacloprid was highly effective against both sucking (Linognathus setosus) and biting (Trichodectes canis) lice over the 6-week period of the trial...

journal_title：Parasitology research

authors： Hanssen I,Mencke N,Asskildt H,Ewald-Hamm D,Dorn H

更新日期：1999-04-01 00:00:00

abstract：:Reverse-transcriptase polymerase chain reaction cloning of the 3'-ends of the alpha-tubulin cDNAs of Tritrichomonas mobilensis in combination with Southern-blot analysis identified seven to eight distinct alpha-tubulin genes. All seven lack a carboxy-terminal tyrosine and the corresponding sequence compatible with pos...

journal_title：Parasitology research

authors： Schneider A,Plessmann U,Felleisen R,Weber K

更新日期：1999-03-01 00:00:00

abstract：:Two neolignans, burchellin and nordihydroguaiaretic acid (NDGA), were toxic only to Trypanosoma cruzi clone Dm28c maintained in brain heart infusion (BHI) medium at a concentration of 100 microg/ml, not 10 microg/ml. When Rhodnius prolixus was fed with epimastigotes of T. cruzi and treated simultaneously with a single...

journal_title：Parasitology research

authors： Cabral MM,Azambuja P,Gottlieb OR,Garcia ES

更新日期：1999-03-01 00:00:00

abstract：:The role of membrane drug-transport mechanisms in resistance to anthelmintics was examined using a flow cytometry method. This method was adapted from assays developed for the study of similar mechanisms in tumor cells. Rhodamine 123, a P-glycoprotein transport probe, associated with the reversal agent verapamil gave ...

journal_title：Parasitology research

authors： Kerboeuf D,Chambrier P,Le Vern Y,Aycardi J

更新日期：1999-02-01 00:00:00

abstract：:The present report demonstrates that the Gymnorhynchus gigas plerocercoid possesses various types of endo- and exoproteases with activity against general (azocoll, azocasein, and azoalbumin) and specific substrates (elastin, keratin, collagen, hemoglobin, fibrinogen, plasma, and immunoglobulin G). The activity against...

journal_title：Parasitology research

authors： Vázquez-López C,de Armas-Serra C,Giménez-Pardo C,Rodríguez-Caabeiro F

更新日期：1999-01-01 00:00:00

abstract：:The morphology of the scolex of the tapeworm Silurotaenica siluri (Batsch, 1786) (Proteocephalidae: Gangesiinae), a specific parasite of European wels (Silurus glanis L.), was studied using light, scanning (SEM), and transmission electron (TEM) microscopy. The scolex of S. siluri is rather uniform in shape and size; i...

journal_title：Parasitology research

authors： Scholz T,Zd'árská Z,de Chambrier A,Drábek R

更新日期：1999-01-01 00:00:00

abstract：:A novel cDNA encoding a lysophospholipase (SmLPLH) homologue has been cloned from the human parasite, Schistosoma mansoni. The predicted primary structure of SmLPLH shares high sequence homology with mammalian lysophospholipases (62%) and also contains aconsensus signature peptide (GXSXG), which is present in all lipa...

journal_title：Parasitology research

authors： Hamdan FF,Ribeiro P

更新日期：1998-10-01 00:00:00

abstract：:Cysticercoids as well as 6-, 10-, and 14-day Hymenolepis diminuta were evaluated in terms of enzymatic activities related to phosphoenolpyruvate (PEP) utilization and mitochondrial succinate accumulation. The data obtained support a transition toward anaerobic electron-transport-dependent succinate accumulation, chara...

journal_title：Parasitology research

authors： Fioravanti CF,Walker DJ,Sandhu PS

更新日期：1998-10-01 00:00:00

abstract：:The effect of pentavalent meglumine antimoniate (glucantime) on the growth kinetics of promastigotes of 13 South American Leishmania strains isolated from patients, sylvatic reservoirs, and vectors was studied. Four of five L. (Viannia) braziliensis human isolates were obtained from drug-responsive patients and one wa...

journal_title：Parasitology research

authors： Moreira ES,Anacleto C,Petrillo-Peixoto ML

更新日期：1998-09-01 00:00:00

abstract：:In schistosomiasis a systemic hyperplasia of the monomacrophagic cell lineage is associated with its mild modifications in myelograms and hemograms. We monitored the in vitro proliferation of myeloid precursors obtained from bone marrow, blood, spleen, and liver. The macrophage colony-forming unit (M-CFU) numbers were...

journal_title：Parasitology research

authors： Dutra HS,El-Cheikh MC,Azevedo SP,Rossi MI,Borojevic R

更新日期：1998-08-01 00:00:00

abstract：:An inexpensive and simple method using acid-phenol was developed for the isolation of total RNA from eimerian oocysts. This procedure provides a pure preparation of undegraded RNA in high yield and can be completed in under 2 h. ...

journal_title：Parasitology research

authors： Johnston DA,Bratty J,Fernando MA

更新日期：1998-07-01 00:00:00

abstract：:A new method for the extraction of DNA from Ascaris suum muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase K allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained...

journal_title：Parasitology research

authors： Di Mito C,Betschart B

更新日期：1998-07-01 00:00:00

abstract：:The purified metalloprotease and the partially purified cysteine protease from pathogenic Cryptobia salmositica were characterized. Using haemoglobin gel electrophoresis, we detected five enzymatic bands in crude parasite lysate; one protease (200 kDa) yielded a metalloprotease band and other four, cysteine protease b...

journal_title：Parasitology research

authors： Zuo X,Woo PT

更新日期：1998-06-01 00:00:00

abstract：:A total of 15 mixtures involving 9 different stocks attributed to the 19/20, 32 and 39 major clonal genotypes of Trypanosoma cruzi were used to infect third-instar nymphs of Triatoma infestans via an artificial feeding device. Three biological parameters were considered: (1) the percentage of infected insects (%II), (...

journal_title：Parasitology research

authors： Pinto AS,de Lana M,Bastrenta B,Barnabé C,Quesney V,Noël S,Tibayrenc M

更新日期：1998-05-01 00:00:00

abstract：:Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a...

journal_title：Parasitology research

authors： Katende J,Morzaria S,Toye P,Skilton R,Nene V,Nkonge C,Musoke A

更新日期：1998-05-01 00:00:00

abstract：:Efforts to derive a physical map of the genome of Eimeria tenella are being made to complement genetic mapping and to assist in the production of an integrated map. A large insert library of DNA from the Houghton (H) reference strain of E. tenella has been constructed in yeast artificial chromosomes (YACs). The librar...

journal_title：Parasitology research

authors： Hoogendoorn B,Toye AA,Bumstead N,Shirley MW

更新日期：1998-01-01 00:00:00

abstract：:The development of Babesia equi was studied in the salivary glands of adult female ticks, Boophilus microplus, using a transmission electron microscope (TEM). Engorged nymphs were obtained from splenectomized foals experimentally infected with B. equi and fed in the adult phase for 5 days on rabbits. Sporogony in B. e...

journal_title：Parasitology research

authors： Guimarães AM,Lima JD,Ribeiro MF,Camargos ER,Bozzi IA

更新日期：1998-01-01 00:00:00

abstract：:Studies of the immune response in chronic helminth infections suggest that parasites modulate the host's immune response. Taenia solium metacestodes, in particular, produce molecules that down-regulate cell-mediated immunity. We have described a small RNA peptide termed metacestode factor (MF) that depresses the murin...

journal_title：Parasitology research

authors： Arechavaleta F,Molinari JL,Tato P

更新日期：1998-01-01 00:00:00

abstract：:Tetramicra brevifilum, a microsporidian parasite of Scophthalmus maximus, was found in Lophius budegassa for the first time. This parasite was detected in 5 of 199 hosts captured in the coastal waters of Barcelona (Northwest Mediterranean), which enlarges the geographic distribution of this microsporidian. Affected fi...

journal_title：Parasitology research

authors： Maíllo PA,Amigó JM,Baena R,Salvadó H,Gracia MP

更新日期：1998-01-01 00:00:00

abstract：:The agar-gel migration technique has previously been described, however, aspects regarding the effect of timing on worm migration needed further scrutiny. In the first experiment, pigs inoculated with Oesophagostomum dentatum were slaughtered simultaneously and their intestines stored at 21-23 degrees C until processe...

journal_title：Parasitology research

authors： Nosal P,Christensen CM,Nansen P

更新日期：1998-01-01 00:00:00

abstract：:In order to identify brain cell types that serve as host cells of Toxoplasma gondii encystation primary cultures from murine brain were infected and stained for neural and parasite stage-specific markers. In mixed culture inoculated with T. gondii tachyzoites, MAP2+ neurons, GFAP+ astrocytes, F4/80+ microglia, and O1+...

journal_title：Parasitology research

authors： Fischer HG,Nitzgen B,Reichmann G,Gross U,Hadding U

更新日期：1997-01-01 00:00:00

abstract：:The induction of protective immunity to Leishmania amazonensis was investigated by injection of parasite clones of low and medium virulence into susceptible mice. To this end, L. amazonensis were cloned by limiting dilution and the clones' virulence was evaluated by the course of infection in susceptible mice. Clones ...

journal_title：Parasitology research

authors： da Fonseca AL,Vallochi AL,Furtado Gde C,Abrahamsohn Ide A,Lima GM

更新日期：1997-01-01 00:00:00

abstract：:A case of orotracheal myiasis in an 80-year-old man in coma for 1 week in the intensive care unit of Baskent University Hospital, Ankara, Turkey, is reported. A number of larvae of Wohlfahrtia magnifica were recovered repeatedly from the mouth and from the intubation tube of the patient and were reared to adults to fa...

journal_title：Parasitology research

authors： Ciftçioğlu N,Altintaş K,Haberal M

更新日期：1997-01-01 00:00:00

abstract：:An easy and rapid tool for the individual identification of Haemonchus species in domestic ruminants might be very useful in the study of natural populations, especially when two (or three) species are sympatric. Techniques based on cytology, cuticular-ridge patterns, sublateral hypodermic chords, or molecular biology...

journal_title：Parasitology research

authors： Jacquiet P,Cabaret J,Cheikh D,Thiam E

更新日期：1997-01-01 00:00:00

abstract：:One of the main drawbacks of experimental amebiasis is the lack of an adequate animal model for invasive intestinal lesions. Mongolian gerbils are useful because both intestinal and hepatic amebiasis can be produced experimentally with Entamoeba histolytica trophozoites. In this paper we show results obtained with in ...

journal_title：Parasitology research

authors： Shibayama M,Navarro-García F,López-Revilla R,Martínez-Palomo A,Tsutsumi V

更新日期：1997-01-01 00:00:00

abstract：:A monoclonal antibody raised by immunization of BALB/c mice with erythrocytic stages of Plasmodium vivax was shown to react with asexual erythrocytic stages of P. chabaudi. The cross-reactivity molecules are antigens of 200 and 148 kDa in P. vivax and of 190 and 70 kDa in P. chabaudi. Immunofluorescence studies of the...

journal_title：Parasitology research

authors： Perez HA,Bracho C,Romano M,De la Rosa M

更新日期：1997-01-01 00:00:00

abstract：:Seventy perch (Perca fluviatilis) caught in Lake Constance at Langenargen in May 1990 were examined for parasites. The frequency distributions of the eyefluke Diplostomum spathaceum (Rudolphi, 1819), and the tapeworms Proteocephalus percae (Müller, 1780) and Triaenophorus nodulosus (Pallas, 1781) fitted closely to a n...

journal_title：Parasitology research

authors： Balling TE,Pfeiffer W

更新日期：1997-01-01 00:00:00

abstract：:Our previous in vivo studies on angiostrongyliasis showed that PF1022A had stronger killing effects against female adults than against males. No killing effects were observed against young adult worms in the central nervous system. To characterize the former in vivo action of PF1022A, in vitro effects of PF1022A on th...

journal_title：Parasitology research

authors： Kachi S,Terada M,Hashimoto H

更新日期：1997-01-01 00:00:00

abstract：:Nitric oxide (NO) production by peritoneal macrophages was evaluated in Calomys callosus and Swiss mice during the course of infection with two strains of Trypanosoma cruzi. In C. callosus, no NO production was detected throughout the period of observation in animals infected with either parasite strain, except for a ...

journal_title：Parasitology research

authors： de Oliveira L,Borges MM,Leal RC,Assreuy J,Kloetzel JK

更新日期：1997-01-01 00:00:00

abstract：:Isometamidium chloride (ISM) is an effective trypanocide with curative and prophylactive activity in husbandry animals. The mode of action of ISM against pathogenic trypanosomes is not fully understood, but there is evidence in the literature that kinetoplastic topoisomerase type II is selectively inhibited by the dru...

journal_title：Parasitology research

authors： Kaminsky R,Schmid C,Lun ZR

更新日期：1997-01-01 00:00:00

abstract：:A study of development of Eimeria tenella in chickens fed high n-3 fatty acids (n-3FA) diets showed ultrastructural degeneration of both asexual and sexual parasite stages. Abnormal shedding of asexual and sexual parasite developmental stages into the cecal lumen was also observed. Ultrastructural degeneration was cha...

journal_title：Parasitology research

authors： Danforth HD,Allen PC,Levander OA

更新日期：1997-01-01 00:00:00

abstract：:The ultrastructure of alpha-bacteroids in relation to the fat-body cells of the lantern bug Pyrops candelaria was described. The fat-body-cell cytoplasm contained numerous mitochondria, rough endoplasmic reticula, vacuoles, and storage granules. Its nucleus had scattered chromatin materials. The alpha-bacteroid was en...

journal_title：Parasitology research

authors： Wang JB,Cheung WW

更新日期：1997-01-01 00:00:00

abstract：:The present study describes a transmission electron microscopical investigation of trophozoites from Giardia lamblia clone GS/M-83-H7 after exposure to monoclonal antibody (MAb) G10/4. From previous studies it is known that this antibody immunoreacts with the parasite's major surface antigen VSP (variable surface prot...

journal_title：Parasitology research

authors： Hemphill A,Stäger S,Gottstein B,Müller N

更新日期：1996-01-01 00:00:00

abstract：:Two isolates of Trypanosoma cruzi were obtained from the patient Berenice, the first human case of Chagas' disease (Chagas 1909), when she was 55 and 71 years old, respectively. The isolates were characterized on the basis of their epimastigote-trypomastigote differentiation in liquid media and of the electrophoretic ...

journal_title：Parasitology research

authors： de Lana M,Chiari CA,Chiari E,Morel CM,Gonçalves AM,Romanha AJ

更新日期：1996-01-01 00:00:00

abstract：:The dynamics of production and excretion of leukotrienes by parasitic larvae of Oesophagostomum dentatum and their role for the development of the larvae were studied. Larvae were cultured in vitro to the fourth stage (L4). Leukotriene B4 (LTB4) was detected in homogenates and in supernatant of larvae, with the homoge...

journal_title：Parasitology research

authors： Daugschies A

更新日期：1996-01-01 00:00:00

abstract：:Topical application and intraperitoneal administration of spiroarsoranes were carried out to cure central nervous system (CNS) trypanosomiasis in the chronic Trypanosoma brucei GVR 35 mouse model. Topical application appeared more efficient than intraperitoneal injection. The periods of aparasitaemia after treatment w...

journal_title：Parasitology research

authors： Loiseau PM,Jennings FW,Lubert P,Wolf JG

更新日期：1996-01-01 00:00:00

abstract：:The localization and distribution of SALMFamide (S1)-like immunoreactivity (IR), was determined at both the cellular and subcellular level in the central nervous system (CNS) of the nematode roundworm Ascaris suum. The techniques of indirect immunofluorescence in conjunction with confocal scanning laser microscopy and...

journal_title：Parasitology research

authors： Brownlee DJ,Fairweather I,Thorndyke MC,Johnston CF

更新日期：1996-01-01 00:00:00

abstract：:A total of 20 decamer primers were used to generate random applied polymorphic DNA (RAPD) markers from 5 isolates of Trypanosoma fallisi, 3 isolates of T. ranarum, 2 isolates of T. rotatorium, and 2 isolates of T. rotatorium-like trypanosomes in addition to 2 species from the American Type Culture Collection, T. chatt...

journal_title：Parasitology research

authors： Lun ZR,Desser SS

更新日期：1996-01-01 00:00:00